mouse anti ranbp2 (Santa Cruz Biotechnology)
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mouse anti ranbp2
Mouse Anti Ranbp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ranbp2+antibody/Ran+BP-2+Antibody/pm41593078-564-33-36
Average 93 stars, based on 44 article reviews
Mouse Anti Ranbp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ranbp2+antibody/Ran+BP-2+Antibody/pm41593078-564-33-36
Average 93 stars, based on 44 article reviews
mouse anti ranbp2 - by Bioz Stars,
2026-10
93/100 stars
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Expressing:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Western Blot:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Imaging:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Staining:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Transfection:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Immunofluorescence:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Fluorescence:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Software:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Purification:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Construct:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Immunoprecipitation:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Activation Assay:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. In Vitro:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Activity Assay:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Binding Assay:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Stable Transfection:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Magnetic Beads:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Clinical Proteomics:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. Membrane:Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.The membranes were then blocked with 5% nonfat milk in TBST for 1 h, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.. Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function. Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti- RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidaseconjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent.. Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function Article Snippet: The membranes were then blocked with 5% nonfat milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer.milk in TBST for 1 hour, followed by overnight incubation at 4 °C with primary antibodies diluted in the same blocking buffer. ... Primary antibodies used in this study are Anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), Anti-RAN polyclonal antibody (Proteintech Group, Inc, Catalog no. 10469-1-AP, 1:3000 dilution), Anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-74518, 1:1000 dilution), Anti-RanGAP1 antibody (Cell Signaling Technology, Catalog no. 36067, 1:3000 dilution), Anti-Rap1 antibody (Santa Cruz Biotechnology, Inc., Catalog no. sc-65, 1:1000 dilution), Anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and Anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).. The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, catalog no. 45-002-401).The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detected using Amersham ECL Prime Western Blotting Detection Reagent (GE.. |
